Generator

Part:BBa_K113007:Design

Designed by: Wu Jingjing,Wang Jinyu   Group: iGEM08_HKUSTers   (2008-10-26)


pBad/araC->T7 polymerase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 3872
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

We used standard assembly to combine pBad/araC and T7 polymerase. The restriction enzymes we used are SpeI and XbaI.


Source

BBa_I0500, iHKU team,

BBa_I712069

References